Microspore culture reveals complex meiotic behaviour in a trigenomic Brassica hybrid
نویسندگان
چکیده
منابع مشابه
Microspore culture reveals high fitness of B. napus-like gametes in an interspecific hybrid between Brassica napus and B. oleracea
The strategies of crossing B. napus with parental species play important role in broadening and improving the genetic basis of B. napus by the introgression of genetic resources from parental species. With these strategies, it is easy to select new types of B. napus, but difficult to select new types of B. rapa or B. oleracea by self-pollination. This characteristic may be a consequence of high...
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The technique of isolated microspore culture for the production of haploid plants is well standardized for many species of Brassica, some of which are important oilseed or vegetable crops. It offers an excellent experimental system for improvement of Brassica crops and study of cell differentiation during shift from gametophytic to sporophytic development. The haploids raised from microspores a...
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Meiotic crossovers (COs) are essential for proper chromosome segregation and the reshuffling of alleles during meiosis. In WT plants, the number of COs is usually small, which limits the genetic variation that can be captured by plant breeding programs. Part of this limitation is imposed by proteins like FANCM, the inactivation of which results in a 3-fold increase in COs in Arabidopsis thalian...
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Calcium is widely known to have a role as a signaling molecule in many different processes, including stress response and activation of the embryogenic program. However, there are no direct clues about calcium levels during microspore embryogenesis, an experimental process that combines a developmental switch toward embryogenesis and the simultaneous application of different stressing factors. ...
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Polyploidization has played an important role in plant evolution and speciation, and newly formed allopolyploids have experienced rapid transcriptomic changes. Here, we compared the transcriptomic differences between a synthetic Brassica allohexaploid and its parents using a high-throughput RNA-Seq method. A total of 35,644,409 sequence reads were generated, and 32,642 genes were aligned from t...
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ژورنال
عنوان ژورنال: BMC Plant Biology
سال: 2015
ISSN: 1471-2229
DOI: 10.1186/s12870-015-0555-9